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c terminal flag angptl2 expression vector  (OriGene)


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    OriGene c terminal flag angptl2 expression vector
    A, Western blot analysis of FLAG-tagged <t>ANGPTL2</t> in the liver (upper panel: lean mice, lower panel: db/db mice). B, Body weight (n = 7–8 mice / group). C, Plasma ANGPTL2 levels 2 week after adenovirus injection (n = 6–8). D, Fasting glucose levels at 2 weeks (n = 7–8 mice / group). E, Glucose tolerance test at 2 weeks after treatment (Left: Lean mice, Right: db/db mice, n = 7–8 mice / group, in Experiment 1). F, Insulin tolerance test 2 weeks after treatment in db/db mice (n = 7–8 mice / group, in Experiment 2). G, Quantitative RT-PCR of mRNAs encoding gluconeogenesis related genes in the liver (Left: Lean mice, Right: db/db mice, n = 8). Data are mean ± SEM, **: P<0.01, *: P<0.05 compared with LacZ group.
    C Terminal Flag Angptl2 Expression Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+terminal+flag+angptl2+expression+vector/ANGPTL2+(NM_012098)+Human+Tagged+ORF+Clone/pmc04489192-55-1-9
    Average 90 stars, based on 1 article reviews
    c terminal flag angptl2 expression vector - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Angiopoietin Like Protein 2 (ANGPTL2) Promotes Adipose Tissue Macrophage and T lymphocyte Accumulation and Leads to Insulin Resistance"

    Article Title: Angiopoietin Like Protein 2 (ANGPTL2) Promotes Adipose Tissue Macrophage and T lymphocyte Accumulation and Leads to Insulin Resistance

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0131176

    A, Western blot analysis of FLAG-tagged ANGPTL2 in the liver (upper panel: lean mice, lower panel: db/db mice). B, Body weight (n = 7–8 mice / group). C, Plasma ANGPTL2 levels 2 week after adenovirus injection (n = 6–8). D, Fasting glucose levels at 2 weeks (n = 7–8 mice / group). E, Glucose tolerance test at 2 weeks after treatment (Left: Lean mice, Right: db/db mice, n = 7–8 mice / group, in Experiment 1). F, Insulin tolerance test 2 weeks after treatment in db/db mice (n = 7–8 mice / group, in Experiment 2). G, Quantitative RT-PCR of mRNAs encoding gluconeogenesis related genes in the liver (Left: Lean mice, Right: db/db mice, n = 8). Data are mean ± SEM, **: P<0.01, *: P<0.05 compared with LacZ group.
    Figure Legend Snippet: A, Western blot analysis of FLAG-tagged ANGPTL2 in the liver (upper panel: lean mice, lower panel: db/db mice). B, Body weight (n = 7–8 mice / group). C, Plasma ANGPTL2 levels 2 week after adenovirus injection (n = 6–8). D, Fasting glucose levels at 2 weeks (n = 7–8 mice / group). E, Glucose tolerance test at 2 weeks after treatment (Left: Lean mice, Right: db/db mice, n = 7–8 mice / group, in Experiment 1). F, Insulin tolerance test 2 weeks after treatment in db/db mice (n = 7–8 mice / group, in Experiment 2). G, Quantitative RT-PCR of mRNAs encoding gluconeogenesis related genes in the liver (Left: Lean mice, Right: db/db mice, n = 8). Data are mean ± SEM, **: P<0.01, *: P<0.05 compared with LacZ group.

    Techniques Used: Western Blot, Injection, Quantitative RT-PCR

    A, Western blotting analysis of FLAG fusion ANGPTL2 protein with anti-FLAG and anti-human Angptl2 antibody (Left: with 2-mercaptoethanol (2-ME), Right: without 2-ME). B, TNF-α concentration in rANGPTL2-treated THP-1 conditioned media (72 hr treatment, n = 3). C, Quantitative RT-PCR of mRNAs encoding Angptl2 and pro-inflammatory related genes in THP-1 cells (24 hr treatment, n = 3). D, Quantitative RT-PCR of mRNAs encoding M2 macrophage markers in THP-1 cells (24 hr treatment, n = 3). Data are mean ± SEM, **: P<0.01, *: P<0.05 compared with BSA group.
    Figure Legend Snippet: A, Western blotting analysis of FLAG fusion ANGPTL2 protein with anti-FLAG and anti-human Angptl2 antibody (Left: with 2-mercaptoethanol (2-ME), Right: without 2-ME). B, TNF-α concentration in rANGPTL2-treated THP-1 conditioned media (72 hr treatment, n = 3). C, Quantitative RT-PCR of mRNAs encoding Angptl2 and pro-inflammatory related genes in THP-1 cells (24 hr treatment, n = 3). D, Quantitative RT-PCR of mRNAs encoding M2 macrophage markers in THP-1 cells (24 hr treatment, n = 3). Data are mean ± SEM, **: P<0.01, *: P<0.05 compared with BSA group.

    Techniques Used: Western Blot, Concentration Assay, Quantitative RT-PCR

    A, Representative images of the liver (Left: LacZ, Right: Angptl2, db/db mice). B, Liver triglyceride levels (Left: Lean mice, Right: db/db mice, n = 7–8 animals per group). C, Representative images of oil red O staining (Left: LacZ, Right: Angptl2, db/db mice). D, Oil red O staining area (n = 7–8, db/db mice). E, Quantitative RT-PCR of mRNAs encoding genes related to fatty acid metabolism in the liver of lean mice (n = 8) F, Quantitative RT-PCR of mRNAs encoding genes related to fatty acid metabolism in the liver of db/db mice (n = 7–8). Data are mean ± SEM, **: P<0.01, *: P<0.05 compared with LacZ group.
    Figure Legend Snippet: A, Representative images of the liver (Left: LacZ, Right: Angptl2, db/db mice). B, Liver triglyceride levels (Left: Lean mice, Right: db/db mice, n = 7–8 animals per group). C, Representative images of oil red O staining (Left: LacZ, Right: Angptl2, db/db mice). D, Oil red O staining area (n = 7–8, db/db mice). E, Quantitative RT-PCR of mRNAs encoding genes related to fatty acid metabolism in the liver of lean mice (n = 8) F, Quantitative RT-PCR of mRNAs encoding genes related to fatty acid metabolism in the liver of db/db mice (n = 7–8). Data are mean ± SEM, **: P<0.01, *: P<0.05 compared with LacZ group.

    Techniques Used: Staining, Quantitative RT-PCR



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    c terminal flag angptl2 expression vector  (OriGene)
    90
    OriGene c terminal flag angptl2 expression vector
    A, Western blot analysis of FLAG-tagged <t>ANGPTL2</t> in the liver (upper panel: lean mice, lower panel: db/db mice). B, Body weight (n = 7–8 mice / group). C, Plasma ANGPTL2 levels 2 week after adenovirus injection (n = 6–8). D, Fasting glucose levels at 2 weeks (n = 7–8 mice / group). E, Glucose tolerance test at 2 weeks after treatment (Left: Lean mice, Right: db/db mice, n = 7–8 mice / group, in Experiment 1). F, Insulin tolerance test 2 weeks after treatment in db/db mice (n = 7–8 mice / group, in Experiment 2). G, Quantitative RT-PCR of mRNAs encoding gluconeogenesis related genes in the liver (Left: Lean mice, Right: db/db mice, n = 8). Data are mean ± SEM, **: P<0.01, *: P<0.05 compared with LacZ group.
    C Terminal Flag Angptl2 Expression Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+terminal+flag+angptl2+expression+vector/ANGPTL2+(NM_012098)+Human+Tagged+ORF+Clone/pmc04489192-55-1-9
    Average 90 stars, based on 1 article reviews
    c terminal flag angptl2 expression vector - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A, Western blot analysis of FLAG-tagged ANGPTL2 in the liver (upper panel: lean mice, lower panel: db/db mice). B, Body weight (n = 7–8 mice / group). C, Plasma ANGPTL2 levels 2 week after adenovirus injection (n = 6–8). D, Fasting glucose levels at 2 weeks (n = 7–8 mice / group). E, Glucose tolerance test at 2 weeks after treatment (Left: Lean mice, Right: db/db mice, n = 7–8 mice / group, in Experiment 1). F, Insulin tolerance test 2 weeks after treatment in db/db mice (n = 7–8 mice / group, in Experiment 2). G, Quantitative RT-PCR of mRNAs encoding gluconeogenesis related genes in the liver (Left: Lean mice, Right: db/db mice, n = 8). Data are mean ± SEM, **: P<0.01, *: P<0.05 compared with LacZ group.

    Journal: PLoS ONE

    Article Title: Angiopoietin Like Protein 2 (ANGPTL2) Promotes Adipose Tissue Macrophage and T lymphocyte Accumulation and Leads to Insulin Resistance

    doi: 10.1371/journal.pone.0131176

    Figure Lengend Snippet: A, Western blot analysis of FLAG-tagged ANGPTL2 in the liver (upper panel: lean mice, lower panel: db/db mice). B, Body weight (n = 7–8 mice / group). C, Plasma ANGPTL2 levels 2 week after adenovirus injection (n = 6–8). D, Fasting glucose levels at 2 weeks (n = 7–8 mice / group). E, Glucose tolerance test at 2 weeks after treatment (Left: Lean mice, Right: db/db mice, n = 7–8 mice / group, in Experiment 1). F, Insulin tolerance test 2 weeks after treatment in db/db mice (n = 7–8 mice / group, in Experiment 2). G, Quantitative RT-PCR of mRNAs encoding gluconeogenesis related genes in the liver (Left: Lean mice, Right: db/db mice, n = 8). Data are mean ± SEM, **: P<0.01, *: P<0.05 compared with LacZ group.

    Article Snippet: Briefly, C-terminal FLAG ANGPTL2 expression vector was obtained from Origene (Myc-DDK-tagged-Human angiopoietin-like 2, RC201917, Rockville, MD).

    Techniques: Western Blot, Injection, Quantitative RT-PCR

    A, Western blotting analysis of FLAG fusion ANGPTL2 protein with anti-FLAG and anti-human Angptl2 antibody (Left: with 2-mercaptoethanol (2-ME), Right: without 2-ME). B, TNF-α concentration in rANGPTL2-treated THP-1 conditioned media (72 hr treatment, n = 3). C, Quantitative RT-PCR of mRNAs encoding Angptl2 and pro-inflammatory related genes in THP-1 cells (24 hr treatment, n = 3). D, Quantitative RT-PCR of mRNAs encoding M2 macrophage markers in THP-1 cells (24 hr treatment, n = 3). Data are mean ± SEM, **: P<0.01, *: P<0.05 compared with BSA group.

    Journal: PLoS ONE

    Article Title: Angiopoietin Like Protein 2 (ANGPTL2) Promotes Adipose Tissue Macrophage and T lymphocyte Accumulation and Leads to Insulin Resistance

    doi: 10.1371/journal.pone.0131176

    Figure Lengend Snippet: A, Western blotting analysis of FLAG fusion ANGPTL2 protein with anti-FLAG and anti-human Angptl2 antibody (Left: with 2-mercaptoethanol (2-ME), Right: without 2-ME). B, TNF-α concentration in rANGPTL2-treated THP-1 conditioned media (72 hr treatment, n = 3). C, Quantitative RT-PCR of mRNAs encoding Angptl2 and pro-inflammatory related genes in THP-1 cells (24 hr treatment, n = 3). D, Quantitative RT-PCR of mRNAs encoding M2 macrophage markers in THP-1 cells (24 hr treatment, n = 3). Data are mean ± SEM, **: P<0.01, *: P<0.05 compared with BSA group.

    Article Snippet: Briefly, C-terminal FLAG ANGPTL2 expression vector was obtained from Origene (Myc-DDK-tagged-Human angiopoietin-like 2, RC201917, Rockville, MD).

    Techniques: Western Blot, Concentration Assay, Quantitative RT-PCR

    A, Representative images of the liver (Left: LacZ, Right: Angptl2, db/db mice). B, Liver triglyceride levels (Left: Lean mice, Right: db/db mice, n = 7–8 animals per group). C, Representative images of oil red O staining (Left: LacZ, Right: Angptl2, db/db mice). D, Oil red O staining area (n = 7–8, db/db mice). E, Quantitative RT-PCR of mRNAs encoding genes related to fatty acid metabolism in the liver of lean mice (n = 8) F, Quantitative RT-PCR of mRNAs encoding genes related to fatty acid metabolism in the liver of db/db mice (n = 7–8). Data are mean ± SEM, **: P<0.01, *: P<0.05 compared with LacZ group.

    Journal: PLoS ONE

    Article Title: Angiopoietin Like Protein 2 (ANGPTL2) Promotes Adipose Tissue Macrophage and T lymphocyte Accumulation and Leads to Insulin Resistance

    doi: 10.1371/journal.pone.0131176

    Figure Lengend Snippet: A, Representative images of the liver (Left: LacZ, Right: Angptl2, db/db mice). B, Liver triglyceride levels (Left: Lean mice, Right: db/db mice, n = 7–8 animals per group). C, Representative images of oil red O staining (Left: LacZ, Right: Angptl2, db/db mice). D, Oil red O staining area (n = 7–8, db/db mice). E, Quantitative RT-PCR of mRNAs encoding genes related to fatty acid metabolism in the liver of lean mice (n = 8) F, Quantitative RT-PCR of mRNAs encoding genes related to fatty acid metabolism in the liver of db/db mice (n = 7–8). Data are mean ± SEM, **: P<0.01, *: P<0.05 compared with LacZ group.

    Article Snippet: Briefly, C-terminal FLAG ANGPTL2 expression vector was obtained from Origene (Myc-DDK-tagged-Human angiopoietin-like 2, RC201917, Rockville, MD).

    Techniques: Staining, Quantitative RT-PCR